Amplification Enzymes
TAQ Polymerase
Ultra-Pure form, Ideal for TA cloning, general PCR applications and SSR genotyping.
Product Description:
Taq DNA polymerase is a thermostable DNA polymerase that is used for the DNA polymerase chain reaction (PCR) in order to amplify DNA sequences. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa
Applications:
PCR, RT-PCR, cDNA synthesis, DNA labelling, Molecular Biology.
| Product Code | MPE- 1L, MPE- 1XL |
| Components | Taq DNA Polymerase at 5U/µl |
| Format | Kit |
| Storage Temperature | -20°C |
| Unit Definition | One unit incorporates 15nmol dNTP into acid-insoluble material in 30 minutes at 75°C |
Hot Start Taq Polymerase
Ideal for routine PCR applications and molecular diagnostics. High specificity and sensitivity.
Product Description:
it is a mixture of taq dna polymerase and an aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal cycling conditions, allowing reactions to be set up at room temperature.
Applications:
PCR, rt-pcr, cdna synthesis, DNA labelling, molecular biology
| Product Code | MPH-M |
| Components | Hot start Taq DNA Polymerase at 5U/µl |
| Format | Kit |
| Storage Temperature | -20°C |
| Unit Definition | One unit incorporates 15nmol dNTP into acid-insoluble material in 30 minutes at 75°C |
Pfu DNA Polymerase
Applicable for the long DNA fragment clone, especially have a great advantage in >10KB fragment amplification, the amplification fragment can be as long as 20 KB.
Product Description:
ILB Genomics pfu fast fidelity dna polymerase is kinds of fast, high amplification efficiency and high-fidelity dna polymerase. This polymerase has the 5′ to 3′ DNA polymerase activity and 3′ to 5′ exonuclease activity.
Applications:
gene cloning, gene expression or mutation analysis
| Product Code | MPE-1S , MPE-2M, MPE-L2 |
| Components | PFU DNA Polymerase with 10X Buffer |
| Format | Kit |
| Storage Temperature | -20°C |
| Unit Definition | One unit incorporates 10nmol dNTP into acid-insoluble material in 30 minutes at 74°C |
Bst Polymerase
Isolated from a recombinant source. Sequencing through problematic secondary structures. Thermophilic DNA polymerase with strong strand displacement activity.
Product Description:
bst dna polymerase from bacillus stearothermophilus is isolated as a recombinant. It lacks the 5´ → 3´ exonuclease domain. Therefore, large fragment of the bst DNA polymerase catalyzes 5′ → 3′ synthesis of DNA and but lacks 5′ → 3′ and 3′ → 5′ exonuclease activities.
Applications:
random-primed DNA labelling, labeling by fill-in 5′-overhangs of dsdna, loop-mediated isothermal amplification (lamp), whole genome amplification (wga).
| Product Code | MPE-B3S1, MPE-B31 |
| Components | Bst DNA Polymerase (Large) with 10X Buffer Sterile water |
| Format | Kit |
| Storage Temperature | -20°C |
| Unit Definition | One unit is defined at the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 65℃. |