DNA Cleanup
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PCR Purification Kit
PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions. It effectively removes primers, dNTPs, unincorporated labeled nucleotides, enzymes, and salts from PCR and other reaction mixtures. The kit can be used for purification of DNA fragments from 25 bp to 20 kb with recovery rates up to 100%. Each purification column has a total binding capacity of up to 25 µg of DNA, and the entire procedure takes 5 minutes. The purified DNA can be used in common downstream applications, such as sequencing, restriction digestion, labeling, ligation, cloning, in vitro transcription, blotting or in situ hybridization.
Agarose Purification kit
For the elution of DNA fragments from agarose gels. The Agarose Gel DNA Extraction Kit provides convenient isolation of high-quality DNA in large amounts. By combining agarose gel electrophoresis and extraction, you can easily concentrate dilute, aqueous DNA solutions. After processing with the kit, the DNA can be recovered in a volume of 20 to 50 μL. Isolated DNA fragments are free of inhibitors that could affect common downstream procedures. For example, recovered DNA can be efficiently ligated into cloning vectors or labeled to high specific activity. Restriction digests proceed without inhibition. Agarose Gel DNA Extraction Kit has been used to extract full-length (CHRNA_v2) and shortest (CHRNA_v3) amplicons from agarose gels. The Agarose DNA Extraction Kit efficiently isolates both small and large DNA fragments from standard or low melting point agarose. Recovered DNA fragments are suitable for ligation and transformation, enzymatic restriction, random primed or nick translation labeling methods, sequencing, PCR/long PCR, cloning, concentrate dilute nucleic acid solutions.