Master Mix
Red Taq Master Mix
Taq DNA polymerase 2x master mix RED is a ready-to-use 2x reaction mix with the taq DNA polymerase, the NH4+ buffer system, dntps and magnesium chloride present. Each reaction requires 25 µl of the 2x master mix RED. Simply add primers, template and water to a total reaction volume of 50 µl to successfully carry out primer extensions and other molecular biology applications.
| Component | Volume/ Reaction | Final Concentration |
| TAQ 2X Master Mix | 25 µl | 1x |
| Primer A | 1 µl | 0.2 µM (0.1 – 1.0 µM) |
| Primer B | 1 µl | 0.2 µM (0.1 – 1.0 µM) |
| DNA | X µl | Genomic DNA: 50ng (10–500 ng) Plasmid DNA: 0.5ng (0.1–1 ng) Bacterial DNA: 5ng (1-10 ng) |
| Water | X µl | |
| Total Volume | 50 µl |
Green Taq Master Mix
Green PCR master mix, 2X is a premixed ready-to-use solution containing bacterially derived taq DNA polymerase, dntps, mgci2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR. The master mix contains two dyes (blue and yellow) that allow monitoring of progress during electrophoresis. Reactions assembled with green master mix have sufficient density for direct loading onto agarose gels.
| Component | Volume/ Reaction | Final Concentration |
| Green PCR 2X Master Mix | 25 µl | 1x |
| Primer A | 1 µl | 0.2 µM (0.1 – 1.0 µM) |
| Primer B | 1 µl | 0.2 µM (0.1 – 1.0 µM) |
| DNA | X µl | Genomic DNA: 50ng (10–500 ng) Plasmid DNA: 0.5ng (0.1–1 ng) Bacterial DNA: 5ng (1-10 ng) |
| Water | X µl | |
| Total Volume | 50 µl |
Pfu Master Mix
PFU fast fidelity DNA polymerase is kinds of fast, high amplification efficiency and high fidelity DNA polymerase. This polymerase has the 5′to 3′DNA polymerase activity and 3′to 5′exonuclease activity. The fidelity of super fi polymerase is the 50 times of the general taq polymerase and the 6 times of the pfu polymerase. The amplification speed is 10-30 sec/KB, which successfully overcome the low amplification efficiency and low amplification speed defect of pfu, reduce much of the reaction time.
| Product Code | |
| Components | 0.1 u/µl Pfu Polymerase 500mM dNTP |
| Format | Kit |
| Storage Temperature | -20°C |
| Unit Definition | One unit incorporates 15nmol dNTP into acid-insoluble material in 30 minutes at 75°C |
Bst Colorimetric Master Mix
The LAMP 2X master mix is a single mix for both colourimetric and real-time fluorescence detection. The LAMP mix is an optimized buffer formulation with BST polymerase and it contains hydroxyl naphthol (HNB) dye is a metal indicator, which enables the visual detection of the mg2+ concentration and its decrease by pyrophosphate precipitation during positive lamp reaction. The addition of HNB to reaction mix changes colour purple to blue in a positive sample during DNA amplification. The real-time fluorescence detection evergreen DNA binding dye (FAM detection channel) used. The mix can be also used any end product LAMP detection and it requires only a heated chamber and sample, which readout of positive amplification analyzed by eye in 30-60 min
| Components | 50 Reactions | 100 Reactions | 200 Reactions |
Reaction Mix
| 1X 800µl | 2X 800µl | 4X 800µl |
| BST polymerase Enzyme | 1X 55µl | 1X 110µl | 1X 220µl |
Hot start 2X Master Mix
APTA hot taq DNA polymerase is a hot-start DNA polymerase using DNA-aptamers instead of antibodies for blocking the activity at ambient temperature. At temperatures above 50°C, the DNA aptamers reversibly dissociate from the enzyme. The polymerase is activated during normal cycling conditions, allowing for a convenient assembly of PCR reactions at room temperature.
| Components | PCR in 50 μl | Final concentration |
2 10x reaction buffer with 25 mM MgCl2 | 5 μl | 1x react. buffer with 1.5 mM MgCl2 |
| PCR dNTP mix (10 mM each ) (Cat. No. P041) | 1 μl | 0.2 mM dNTP each |
| 5´ primer (50 μM) | 0.5 μl | 0.5 μM |
| 3´ primer (50 μM) | 0.5 μl | 0.5 μM |
| Aptame-Taq DNA polymerase (1U/μl) | 2.5 ul | 2.5 U (0.05 U/μl) |
| Template DNA (1 ng/μl – 1 μg/μl) | 1 ul | 0.02 ng/μl – 0.02 μg/μl |
| PCR H2O (Cat. No. P042) | 39.5 ul |