One-Step RT-qPCR
RT-qPCR Probe Master
One-step RT-qPCR Probe Master Mix is an optimized and highly efficient reaction mixture developed for first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The master mix, formulated as a 2× reaction mixture, contains all components necessary for both cDNA synthesis and real-time PCR (including enzymes, dNTPs, stabilizers and enhancers), except primers, probes and RNA template. This master mix enables fast and highly reproducible procedures on the most common real-time PCR apparatus, from either total RNA or mRNA, and it was specifically developed for probe-detection technology. The latest developments in PCR enhancers have been incorporated in the One-step RT-qPCR Master Mix, including buffer chemistry and incorporation of highly robust engineered enzymes.
Applications
Robust qpcr mix for high sample volume input up to 10 ul
- Rapid
- Ultra-sensitive
- Developed for probe-detection technology
- Unique buffer chemistry and balanced mixture of enzymes (includes RNase inhibitor)
- Does not contain any passive reference dye.
RT-qPCR Probe Master UNG
RT-qPCR Probe Master UNG is designed for quantitative real-time analyses of RNA templates using Dual Labelled Fluorescent Probes. The ready-to-use mix is based on a genetically engineered reverse transcriptase with enhanced thermal stability providing increased specificity, high cDNA yield and improved efficiency for highly structured and long cDNA fragments.
The 2x conc. mix contains all reagents required for RT-qPCR (except template, primers and the dual labelled fluorescent probe) to ensure fast and easy preparation with a minimum of pipetting steps. The premium quality enzymes and the optimized reaction buffer containing ultrapure dNTPs ensure superior real time PCR results. The mix contains UNG (Uracil-N-Glycosylase) and dUTP instead of dTTP to eliminate carry-over contamination of DNA from previous PCR reactions. The UNG treatment at the onset of thermal cycling removes uracil residues from dU-containing DNA and prevents it from serving as template.
Applications
Gene expression analysis
- DNA / RNA target detection
- miRNA profiling / quantification
- Copy number variation (CNV) analysis.
RT-qPCR Probe Master high ROX
Hot start protocols have been shown to significantly improve the specificity, sensitivity, and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of Taq DNA Polymerase make the modified enzyme inactive at room temperature. Therefore, there is no elongation during the period when primers can non-specifically bind. Taq DNA Polymerase is activated by removing the blocking groups at a high temperature (i.e., a preincubation step at +95°C).
Universal ready-to-use hot start reaction mix for qPCR and RT-qPCR on all real-time PCR systems requiring normalization with ROX. Universal Probe Master (Rox) includes a novel reference dye that enables its use on all real-time PCR instruments requiring normalization with ROX, without modification or adjustments to the specific instrument or protocol. This ready-to-use, 2x concentrated master mix contains all reagents (except primers, probe, and template) needed for running quantitative, real-time DNA-detection assays, including qPCR and two-step qRT-PCR, in the hydrolysis probe detection format.
Applications
In quantitative real-time polymerase chain reaction (qRT-PCR) reactions for the quantification of endogenous miRNAs.
In reverse transcriptase (RT-PCR) to study tumor necrosis factor (TNF) expression in whole synovial tissue of undifferentiated peripheral inflammatory arthritis (UPIA) patients
For the amplification and detection of any DNA or cDNA target, including those that are GC- or AT-rich by quantitative PCR.
RT-qPCR Sybr Master
The one-step RT-PCR kit designed for measurement of gene expression. This convenient 2X ready mix includes M-MLV RT, SYBR Green I dye, Taq DNA polymerase, 99% pure deoxynucleotides, buffer, glass passivator, and stabilizers. The Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70°C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques.
Applications
For RNA quantification, and to detect mRNA expression levels. The SYBR Green Quantitative RT-PCR kit provides a highly sensitive method for the quantitative analysis of gene expression.
RT-qPCR Sybr Master high ROX
SYBR Green High ROX quantitative RT-PCR mixes are made for use with qPCR devices requiring high ROX as an internal reference dye for fluorescent signal normalization and correction of optical well-to-well variations. The SYBR Green mix consists of all the necessary components (except template DNA and primers): a Reverse transcriptase, a Taq polymerase, dNTPs, MgCl2, SYBR Green and High ROX dye.
Applications
Nucleic acid amplification and expression profiling
- Gene expression analysis
- Genomics-related applications
- Genotyping
- Mutation detection
- Pathogen detection
RT-qPCR Green Master
qPCR Green Master is designed for quantitative real-time analysis of DNA samples including High Resolution Melting (HRM) curve analysis. The mix contains all reagents required for qPCR (except template and primers) in a premixed 2x concentrated ready-to-use solution. It is recommended for routine PCR applications, high throughput PCR or genotyping and provides an improved specificity and sensitivity when amplifying low-copy-number targets or working with complex backgrounds.
The mix is based on an optimized hot-start polymerase. Its activity is blocked by antibody at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of non-specifically annealed primers and primer-dimer formation at low temperatures during PCR setup.
The fluorescent DNA stain intercalates into the amplification product during the PCR process and allows the direct quantification of target DNA without the need to synthesize sequence-specific labelled probes.
Applications
For RNA quantification, and to detect mRNA expression levels. The SYBR Green Quantitative RT-PCR kit provides a highly sensitive method for the quantitative analysis of gene expression.
RT-qPCR Green Master high ROX
qPCR Green Master is designed for quantitative real-time analysis of DNA samples including High Resolution Melting (HRM) curve analysis. The mix contains all reagents required for qPCR (except template and primers) in a premixed 2x concentrated ready-to-use solution. It is recommended for routine PCR applications, high throughput PCR or genotyping and provides an improved specificity and sensitivity when amplifying low-copy-number targets or working with complex backgrounds.
The mix is based on an optimized hot-start polymerase. Its activity is blocked by antibody at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of non-specifically annealed primers and primer-dimer formation at low temperatures during PCR setup.
The fluorescent DNA stain intercalates into the amplification product during the PCR process and allows the direct quantification of target DNA without the need to synthesize sequence-specific labelled probes.