Reverse Transcriptase
cDNA Synthesis Kit
The use of RT for first-strand cDNA synthesis has certain advantages. The higher thermostability of this enzyme compared to M-MuLV RT allows the reaction to be performed at +42°C, giving higher specificity and better resolution of secondary structures compared to a reaction performed at +37°C.
The First Strand cDNA Synthesis Kit is used for the synthesis of the first strand cDNA as the starting reaction for two-step RT PCR. The kit includes Reverse Transcriptase for first strand synthesis, two different primers, our PCR Nucleotide Mix, and Control Neo pa RNA. PCR products that are generated by RT-PCR can be cloned using standard procedures. The amplification of RNA requires the conversion of the RNA substrate into DNA. This is achieved by a reverse transcriptase. The resulting cDNA can be used as a template for a standard PCR.
Thermostable reverse Transcriptase
Used to transcribe RNA into DNA and facilitates efficient mRNA template driven synthesis of cDNAs. This is due to the ability of this enhanced RT to transcribe large mRNA templates, to transcribe through difficult secondary structures, and to detect low abundance mRNAs by RT-PCR. Reverse transcriptase catalyses RNA template incorporation of dNTPs into complimentary DNA through phosphodiester bond formation.
Applications
Greater sensitivity for low abundance mRNA
Unsurpassed transcription through difficult secondary structures at elevated temperatures (up to 65°C)
Efficient generation of full-length cDNA, up to 14.1 kb.