Supplements
RNASE Inhibitor – Recombinant
RNase Inhibitor inactivates a wide spectrum of RNases, including
- RNase A
- RNase B
- RNase T2
Thus, Protector RNase Inhibitor can help prevent RNase degradation in any application where RNases could cause problems. For instance, it can: protect mRNA during cDNA synthesis reactions, RT-PCR (in conventional thermal cyclers and qPCR systems), or in vitro transcription/translation reactions, protect viral RNA during in vitro virus replication, inhibit RNases during RNA isolation and purification and can be used in RNase protection assays. It helps prepare RNase-free antibodies.
Thermolabile UNG (Uracil N-Glycosylase)
Uracil-DNA Glycosylase, heat-labile contains the equally named enzyme found in the marine bacterium BMTU 3346. Like the UNG from E. coli it hydrolyzes uracil-glycosidic bonds in single- or double-stranded DNA, excising uracil and creating alkali-sensitive a basic site in the DNA. These a basic site can be hydrolysed by endonuclease, heat, or alkali treatment. Depending on how the DNA is prepared, Uracil-DNA Glycosylase can be used to achieve general, site-specific, or strand-specific U-DNA cleavage. This enzyme shows lower thermostability and is therefore easier to inactivate.
Specificity
- Uracil-DNA glycosylase hydrolyses uracil-glycosidic bonds at U-DNA sites in single- and double stranded DNA, excising uracil and creating alkali sensitive a basic site in the DNA.
- The enzyme is active on both single-stranded DNA and double-stranded DNA.
- Uracil-DNA glycosylase is inactive on RNA and native, uracil-free DNA.
- Since uracil-DNA glycosylase has no metal ion requirements, it is fully active in the presence of EDTA.